Investigation of native fluorescence spectral difference among prostate cancer cell lines with different risk levels

Yang Pu; Jianpeng Xue; Baogang Xu; Wubao Wang; Yueqing Gu; Rui Tang; S. Achilefu; Ellen Ackerstaff; Jason A. Koutcher; R. R. Alfano

doi:10.1117/12.2001443

19 March 2013 Investigation of native fluorescence spectral difference among prostate cancer cell lines with different risk levels

Yang Pu, Jianpeng Xue, Baogang Xu, Wubao Wang, Yueqing Gu, Rui Tang, S. Achilefu, Ellen Ackerstaff, Jason A. Koutcher, R. R. Alfano

Author Affiliations +

Proceedings Volume 8577, Optical Biopsy XI; 85770X (2013) https://doi.org/10.1117/12.2001443
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

The alteration of native fluorophores among different types of cancer cell lines was investigated by the fluorescence spectroscopy. Different types of cancer cell lines with different risk levels, such as moderate metastatic (DU-145) and advanced metastatic (PC-3) cell lines as well as normal cell line (Fibroblast), were excited by the selective excitation wavelength of 300 nm to explore changes of the relative contents of tryptophan and NADH using principal component analysis (PCA). The higher relative content of tryptophan was observed in the advanced metastatic cancer cell lines in comparison with the moderate metastatic and non aggressive cell lines.

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Yang Pu, Jianpeng Xue, Baogang Xu, Wubao Wang, Yueqing Gu, Rui Tang, S. Achilefu, Ellen Ackerstaff, Jason A. Koutcher, and R. R. Alfano "Investigation of native fluorescence spectral difference among prostate cancer cell lines with different risk levels", Proc. SPIE 8577, Optical Biopsy XI, 85770X (19 March 2013); https://doi.org/10.1117/12.2001443

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