Numerical and experimental studies of x-ray luminescence optical tomography for small animal imaging

Changqing Li; Arnulfo Martínez Dávalos; Simon R. Cherry

doi:10.1117/12.2004770

25 March 2013 Numerical and experimental studies of x-ray luminescence optical tomography for small animal imaging

Changqing Li, Arnulfo Martínez Dávalos, Simon R. Cherry

Proceedings Volume 8578, Optical Tomography and Spectroscopy of Tissue X; 85781B (2013) https://doi.org/10.1117/12.2004770
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

X-ray luminescence optical tomography (XLOT) is an emerging hybrid imaging modality in which x-ray excitable particles (phosphor particles) emit optical photons when stimulated with a collimated x-ray beam. XLOT combines the high sensitivity of optical imaging with the high spatial resolution of x-ray imaging. For XLOT reconstruction, we compared two reconstruction algorithms, conventional filtered backprojection (FBP) and a new algorithm, x-ray luminescence optical tomography with excitation priors (XLOT-EP), in which photon propagation is modeled with the diffusion equation and the x-ray beam positions are used as reconstruction priors. Numerical simulations based on dose calculations are used to validate the proposed XLOT imaging system and the reconstruction algorithms. Simulation results show that XLOT can better detect inclusions of particles than x-ray computed tomography (CT) alone. Nanoparticle concentrations reconstructed with XLOT-EP are much less dependent on target depths than those obtained with FBP. Measurements at just two orthogonal projections are sufficient for the XLOT-EP to reconstruct an XLOT image for simple source distributions. The heterogeneity of x-ray dose distribution is included in the XLOT-EP reconstruction and improves the reconstruction accuracy, suggesting that there is a need to calculate the x-ray dose distribution for experimental XLOT imaging. We have built a prototype XLOT imaging system, in which a collimated x-ray bean is used to scan the samples and an electron multiplying charge couple device (EMCCD) is used to collect the optical photons emitted by the x-ray excitable particles. Phantom experiments have been performed to validate the XLOT system and the proposed reconstruction algorithms.

Citation Download Citation

Changqing Li, Arnulfo Martínez Dávalos, and Simon R. Cherry "Numerical and experimental studies of x-ray luminescence optical tomography for small animal imaging", Proc. SPIE 8578, Optical Tomography and Spectroscopy of Tissue X, 85781B (25 March 2013); https://doi.org/10.1117/12.2004770

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available