Förster resonance energy transfer photoacoustic microscopy

Yu Wang; Lihong V. Wang

doi:10.1117/12.2004150

4 March 2013 Förster resonance energy transfer photoacoustic microscopy

Yu Wang, Lihong V. Wang

Proceedings Volume 8581, Photons Plus Ultrasound: Imaging and Sensing 2013; 85812E (2013) https://doi.org/10.1117/12.2004150
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Förster resonance energy transfer (FRET) provides fluorescence signals sensitive to intra- and inter-molecular distances in the 1-10 nm range. Widely applied in the optical imaging environment, FRET enables visualization of physicochemical processes in molecular interactions and conformation changes. We reported photoacoustic imaging of FRET, based on non-radiative decay that produces heat and subsequent acoustic waves. The experimental results show that photoacoustic imaging offers better penetration into scattering biological tissue. Through its ability to three-dimensionally image tissue with scalable resolution, photoacoustic microscopy provides a beneficial biomedical tool to broaden the in vivo application of the FRET technique.

Citation Download Citation

Yu Wang and Lihong V. Wang "Förster resonance energy transfer photoacoustic microscopy", Proc. SPIE 8581, Photons Plus Ultrasound: Imaging and Sensing 2013, 85812E (4 March 2013); https://doi.org/10.1117/12.2004150

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