Determining the influence of age and diabetes on the second-harmonic generation strength of dermal collagen fibers in vivo by using electronic noises

Wei-Chun Hung; Chi-Kuang Sun; Argon Chen

doi:10.1117/12.2000993

22 February 2013 Determining the influence of age and diabetes on the second-harmonic generation strength of dermal collagen fibers in vivo by using electronic noises

Wei-Chun Hung, Chi-Kuang Sun, Argon Chen

Author Affiliations +

Proceedings Volume 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI; 85871R (2013) https://doi.org/10.1117/12.2000993
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

It is commonly believed that intrinsic skin aging is associated with the change of the collagen structures. The influence of the diabetes on the skin collagen is also considered to be similar to aging. Moreover, second-harmonic-generation (SHG) in collagen fibers is known to reflect the detailed collagen structures. It is thus highly valuable to adopt the SHG intensity as a collagen structure indicator. With the help of SHG, recently one can achieve in vivo imaging which provides the information of what really happens beneath the human skin. However, when analyzing the images, the SHG brightness of each pixel highly depends on the illumination condition, the depth of the SHG source, and the voltage of PMT. Therefore, it is important to calibrate these factors before statistical analysis. In this paper, we present our recent development that calibrates the in vivo SHG images by using noises. We first determine the regions of signals and noises by setting a threshold relating to the standard deviation of the image. By using the assumption that the noise was amplified by PMT with an amplification ratio the same as the SHG signal, we can define the brightness of the noise region as a parameter representing the voltage of PMT, and use this parameter to calibrate all SHG images. After calibrating, we can then compare different images from volunteers and analyze the influence of aging and diabetes on the SHG intensity from collagen fibers, even if the voltage of PMT was not fixed.

Citation Download Citation

Wei-Chun Hung, Chi-Kuang Sun, and Argon Chen "Determining the influence of age and diabetes on the second-harmonic generation strength of dermal collagen fibers in vivo by using electronic noises", Proc. SPIE 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI, 85871R (22 February 2013); https://doi.org/10.1117/12.2000993

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
5 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Collagen

Second-harmonic generation

Interference (communication)

Calibration

Skin

In vivo imaging

Signal to noise ratio

Show All Keywords

Keywords/Phrases

Search In:

Publication Years