Imaging plant tissues with coherent Raman scattering microscopy

J. Moger; J. C. Mansfield

doi:10.1117/12.2001696

22 February 2013 Imaging plant tissues with coherent Raman scattering microscopy

J. Moger, J. C. Mansfield

Proceedings Volume 8588, Multiphoton Microscopy in the Biomedical Sciences XIII; 85880I (2013) https://doi.org/10.1117/12.2001696
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Microscopic chemical mapping of living plant tissues without the use of extrinsic labels would represent a major advance in analytical capability for many areas of biological research; Coherent Raman Scattering (CRS) microscopy offers label-free chemical imaging based on vibrational spectroscopy and is an obvious solution. However, due to the high levels of optical absorption and fluorescent emission in plant tissues the technique is severely limited for in-vivo plant imaging. This paper reports preliminary results regarding the technical issues associated with performing label-free imaging in plant tissues with CRS and discusses how they may be mitigated in future applications.

Citation Download Citation

J. Moger and J. C. Mansfield "Imaging plant tissues with coherent Raman scattering microscopy", Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 85880I (22 February 2013); https://doi.org/10.1117/12.2001696

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