Two-photon excited endogenous fluorescence for label-free in vivo imaging ingestion of disease-causing bacteria by human leukocytes

Yan Zeng; Bo Yan; Qiqi Sun; Seng Khoon Teh; Wei Zhang; Zilong Wen; Jianan Y. Qu

doi:10.1117/12.2002768

22 February 2013 Two-photon excited endogenous fluorescence for label-free in vivo imaging ingestion of disease-causing bacteria by human leukocytes

Yan Zeng, Bo Yan, Qiqi Sun, Seng Khoon Teh, Wei Zhang, Zilong Wen, Jianan Y. Qu

Author Affiliations +

Proceedings Volume 8588, Multiphoton Microscopy in the Biomedical Sciences XIII; 85881L (2013) https://doi.org/10.1117/12.2002768
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Real time and in vivo monitoring leukocyte behavior provides unique information to understand the physiological and pathological process of infection. In this study, we demonstrate that two-photon excited reduced nicotinamide adenine dinucleotide (NADH) fluorescence provides imaging contrast to distinguish granulocyte and agranulocyte. By using spectral and time-resolved NADH fluorescence, we study the immune response of human neutrophils against bacterial infection (Escherichia coli). The two-photon excited NADH fluorescence images clearly review the morphological changes from resting neutrophils (round shape) to activated neutrophils (ruffle shape) during phagocytosis. The free-tobound NADH ratio of neutrophils decreases after ingesting disease-causing pathogen: Escherichia coli. This finding may provide a new optical tool to investigate inflammatory processes by using NADH fluorescence in vivo.

Citation Download Citation

Yan Zeng, Bo Yan, Qiqi Sun, Seng Khoon Teh, Wei Zhang, Zilong Wen, and Jianan Y. Qu "Two-photon excited endogenous fluorescence for label-free in vivo imaging ingestion of disease-causing bacteria by human leukocytes", Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 85881L (22 February 2013); https://doi.org/10.1117/12.2002768

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KEYWORDS

Luminescence

Blood

In vivo imaging

Mode conditioning cables

Molecules

Pathogens

Bacteria

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