3D quantitative Fourier analysis of second harmonic generation microscopy images of collagen structure in cartilage

Elisabeth I. Romijn; Magnus B. Lilledahl

doi:10.1117/12.2007224

22 February 2013 3D quantitative Fourier analysis of second harmonic generation microscopy images of collagen structure in cartilage

Elisabeth I. Romijn, Magnus B. Lilledahl

Author Affiliations +

Proceedings Volume 8588, Multiphoton Microscopy in the Biomedical Sciences XIII; 85881Y (2013) https://doi.org/10.1117/12.2007224
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

One of the main advantages of nonlinear microscopy is that it provides 3D imaging capability. Second harmonic generation is widely used to image the 3D structure of collagen fibers, and several works have highlighted the modification of the collagen fiber fabric in important diseases. By using an ellipsoidal specific fitting technique on the Fourier transformed image, we show, using both synthetic images and SHG images from cartilage, that the 3D direction of the collagen fibers can be robustly determined.

Citation Download Citation

Elisabeth I. Romijn and Magnus B. Lilledahl "3D quantitative Fourier analysis of second harmonic generation microscopy images of collagen structure in cartilage", Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 85881Y (22 February 2013); https://doi.org/10.1117/12.2007224

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