Clinical multiphoton endoscopy with FLIM capability

Martin Weinigel; Hans Georg Breunig; Peter Fischer; Marcel Kellner-Höfer; Rainer Bückle; Karsten König

doi:10.1117/12.2001849

22 February 2013 Clinical multiphoton endoscopy with FLIM capability

Martin Weinigel, Hans Georg Breunig, Peter Fischer, Marcel Kellner-Höfer, Rainer Bückle, Karsten König

Proceedings Volume 8588, Multiphoton Microscopy in the Biomedical Sciences XIII; 85882E (2013) https://doi.org/10.1117/12.2001849
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Multiphoton endoscopy can be applied for intra-corporeal imaging as well as to examine otherwise hard-to-access tissue areas like chronic wounds. Using high-NA (NA = 0.8) gradient-index (GRIN) lens-based endoscopes with a diameter of 1.4 mm and effective lengths of 7 mm and 20 mm, respectively, two-photon excitation of endogenous fluorophores and second-harmonic generation (SHG) is used for multimodal in vivo imaging of human skin. A further imaging modality is fluorescence lifetime imaging (FLIM) which allows functional imaging to investigate the healing mechanism of chronic wounds and the corresponding cell metabolism. We performed first in vivo measurements using FLIM endoscopy with the medically-certified multiphoton tomograph MPTflex^® in combination with a computer-controlled motorized scan head and a GRIN-lens endoscope.

Citation Download Citation

Martin Weinigel, Hans Georg Breunig, Peter Fischer, Marcel Kellner-Höfer, Rainer Bückle, and Karsten König "Clinical multiphoton endoscopy with FLIM capability", Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 85882E (22 February 2013); https://doi.org/10.1117/12.2001849

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