In vivo imaging of dermal collagen in skin burn by collagen-sensitive second-harmonic-generation microscopy

Takeshi Yasui; Ryosuke Tanaka; Eiji Hase; Shu-ichiro Fukushima; Tsutomu Araki

doi:10.1117/12.2002927

22 February 2013 In vivo imaging of dermal collagen in skin burn by collagen-sensitive second-harmonic-generation microscopy

Takeshi Yasui, Ryosuke Tanaka, Eiji Hase, Shu-ichiro Fukushima, Tsutomu Araki

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Proceedings Volume 8588, Multiphoton Microscopy in the Biomedical Sciences XIII; 85882W (2013) https://doi.org/10.1117/12.2002927
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Optical assessment of skin burns is possible with second-harmonic-generation (SHG) microscopy due to its high sensitivity to thermal denaturation of collagen molecules. In contrast to previous studies that were performed using excised tissue specimens ex vivo, in this study, we demonstrated in vivo observation of dermal collagen fibers in living rat burn models with SHG microscopy. We confirmed that changes in SHG vanishing patterns in the SHG images depended on the burn degree. The results imply that SHG microscopy can be used as a low-invasiveness, highly quantitative tool for skin burn assessment.

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Takeshi Yasui, Ryosuke Tanaka, Eiji Hase, Shu-ichiro Fukushima, and Tsutomu Araki "In vivo imaging of dermal collagen in skin burn by collagen-sensitive second-harmonic-generation microscopy", Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 85882W (22 February 2013); https://doi.org/10.1117/12.2002927

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KEYWORDS

Second-harmonic generation

Collagen

Skin

Microscopy

In vivo imaging

Tissue optics

Molecules

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