Plasmon-enhanced emission from single fluorescent proteins

Jessica E. Donehue; Beth L. Haas; Esther Wertz; Courtney N. Talicska; Julie S. Biteen

doi:10.1117/12.2002062

21 February 2013 Plasmon-enhanced emission from single fluorescent proteins

Jessica E. Donehue, Beth L. Haas, Esther Wertz, Courtney N. Talicska, Julie S. Biteen

Proceedings Volume 8597, Plasmonics in Biology and Medicine X; 85970J (2013) https://doi.org/10.1117/12.2002062
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

In this work, we use evaporated gold nanoparticle films (GNPFs) as substrates for plasmon-enhanced imaging of two fluorescent proteins (FPs): mCherry and YFP. Through single-molecule epifluorescence microscopy, we show enhancement of single FP emission in the presence of GNPFs. The gold-coupled FPs demonstrate emission up to four times brighter and seven times longer lived, yielding order-of-magnitude enhancements in total photons detected. Ultimately, this results in increased localization accuracies for single-molecule imaging. Furthermore, we introduce preliminary results for enhancement of mCherry-labeled TcpP membrane proteins inside live Vibrio cholerae cells coupled to GNPFs. Our work indicates that plasmonic substrates are uniquely advantageous for super-resolution imaging and that plasmon-enhanced imaging is a promising technique for improving live cell single-molecule microscopy.

Citation Download Citation

Jessica E. Donehue, Beth L. Haas, Esther Wertz, Courtney N. Talicska, and Julie S. Biteen "Plasmon-enhanced emission from single fluorescent proteins", Proc. SPIE 8597, Plasmonics in Biology and Medicine X, 85970J (21 February 2013); https://doi.org/10.1117/12.2002062

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