Laser guidance in a microfluidic biochip

Wan Qin; Lucas Schmidt; Xiaoqi Yang; Lina Wei; Ting Huang; Julie X. Yuan; Zhen Ma; Xiang Peng; Xiaocong Yuan; Bruce Z. Gao

doi:10.1117/12.2003255

9 March 2013 Laser guidance in a microfluidic biochip

Wan Qin, Lucas Schmidt, Xiaoqi Yang, Lina Wei, Ting Huang, Julie X. Yuan, Zhen Ma, Xiang Peng, Xiaocong Yuan, Bruce Z. Gao

Author Affiliations +

Proceedings Volume 8615, Microfluidics, BioMEMS, and Medical Microsystems XI; 86150S (2013) https://doi.org/10.1117/12.2003255
Event: SPIE MOEMS-MEMS, 2013, San Francisco, California, United States

Abstract

In this study, we developed a microfluidic biochip to perform laser guidance on two cell types, chick embryonic forebrain neurons and chick embryonic spinal cord neurons. The neurons we obtained from these two cell types have no difference in morphology as observed under a high-magnification microscope. However, when flowing in the microfluidic channel and simultaneously being laser-guided, the two cell types gained quite different guidance velocities under the same experimental conditions. The experimental results demonstrate that different cell types with the same morphology (e.g., size, shape, etc.) can be effectively distinguished from each other by measuring the difference of guidance velocities (the maximum flow velocities minus the initial flow velocities). This technique is expected to provide a new approach to high-throughput, label-free cell sorting with sensitivity.

Citation Download Citation

Wan Qin, Lucas Schmidt, Xiaoqi Yang, Lina Wei, Ting Huang, Julie X. Yuan, Zhen Ma, Xiang Peng, Xiaocong Yuan, and Bruce Z. Gao "Laser guidance in a microfluidic biochip", Proc. SPIE 8615, Microfluidics, BioMEMS, and Medical Microsystems XI, 86150S (9 March 2013); https://doi.org/10.1117/12.2003255

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KEYWORDS

Microfluidics

Particles

Neurons

Geometrical optics

CMOS cameras

Biomedical optics

Laser scattering

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