Color contrast of red blood cells on solid substrate

Adkham A. Paiziev

doi:10.1117/12.2018452

26 February 2013 Color contrast of red blood cells on solid substrate

Adkham A. Paiziev

Proceedings Volume 8699, Saratov Fall Meeting 2012: Optical Technologies in Biophysics and Medicine XIV; and Laser Physics and Photonics XIV; 869908 (2013) https://doi.org/10.1117/12.2018452
Event: Saratov Fall Meeting and Workshop on Laser Physics and Photonics 2012, 2012, Saratov, Russian Federation

Abstract

In present study we developed the new method of colour visualization of red blood cells without using any chemical staining. The method based on physical phenomena a white light interference on thin transparent films. It is shown that in the case of thin human blood smears colour interference contrast occurs on solid polished substrates. The best contrast shows substrates with maximal refractive index (Mo, W, Si). These materials have been selected as substrate instead of ordinary microscopic slide in reflected light microscopy. It is shown that reflection of incident white light from blood cell surface and boundary cell-substrate generate two coherent lights. The second one (object signal) after passing through red blood cell gathers additional phase and after interference interaction with reference signal (light reflected from outer cell surface) enables cell image in colour. Number of blood smears of healthy persons (control) and patients who were diagnosed with cancer are presented. It is concluded that the offered method may be used as an effective diagnostic tool to detect early stage blood cells lesion by its interference painting in white light. Offered method may be used in research laboratories, hospitals, diagnostic centres, emergency medicine and other as complementary diagnostic tool to present convenient optical and electron microscopy technique.

Citation Download Citation

Adkham A. Paiziev "Color contrast of red blood cells on solid substrate", Proc. SPIE 8699, Saratov Fall Meeting 2012: Optical Technologies in Biophysics and Medicine XIV; and Laser Physics and Photonics XIV, 869908 (26 February 2013); https://doi.org/10.1117/12.2018452

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KEYWORDS

Blood

Microscopes

Refractive index

Silicon

Microscopy

Reflectivity

Diagnostics

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