Comparison between optical measurements made from natural and frozen samples at optical clearing

Luís Oliveira; Maria Inês Carvalho; Elisabete Nogueira; Valery V. Tuchin

doi:10.1117/12.2016936

26 February 2013 Comparison between optical measurements made from natural and frozen samples at optical clearing

Luís Oliveira, Maria Inês Carvalho, Elisabete Nogueira, Valery V. Tuchin

Proceedings Volume 8699, Saratov Fall Meeting 2012: Optical Technologies in Biophysics and Medicine XIV; and Laser Physics and Photonics XIV; 86990A (2013) https://doi.org/10.1117/12.2016936
Event: Saratov Fall Meeting and Workshop on Laser Physics and Photonics 2012, 2012, Saratov, Russian Federation

Abstract

To determine the differences between the optical clearing effects created by ethylene glycol in fresh and frozen samples, we have performed several measurements from samples in both conditions. Fresh samples were used after animal sacrifice and frozen samples were kept at -20°C for 72 hours. The different measurements performed with samples from both cases were total transmittance, collimated transmittance, total reflectance and specular reflectance. Considering, for instance, collimated transmittance measurements, we have verified that the spectra measured from both samples before adding the solution present different levels of collimated transmittance. The time-dependence evolution of the collimated transmittance spectrum is similar between both cases of samples, but since they present different levels of “natural” transmittance, the optical clearing effect is observed at different levels if we compare between fresh and frozen samples.

Citation Download Citation

Luís Oliveira, Maria Inês Carvalho, Elisabete Nogueira, and Valery V. Tuchin "Comparison between optical measurements made from natural and frozen samples at optical clearing", Proc. SPIE 8699, Saratov Fall Meeting 2012: Optical Technologies in Biophysics and Medicine XIV; and Laser Physics and Photonics XIV, 86990A (26 February 2013); https://doi.org/10.1117/12.2016936

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KEYWORDS

Optical clearing

Transmittance

Reflectivity

Absorbance

Technetium

Collimation

Tissue optics

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