Diffuse reflectance spectroscopy and optical polarization imaging of in-vivo biological tissue

A. Mora-Núñez; Y. Castillejos; G. García-Torales; G. Martínez-Ponce

doi:10.1117/12.2025878

18 November 2013 Diffuse reflectance spectroscopy and optical polarization imaging of in-vivo biological tissue

A. Mora-Núñez, Y. Castillejos, G. García-Torales, G. Martínez-Ponce

Proceedings Volume 8785, 8th Iberoamerican Optics Meeting and 11th Latin American Meeting on Optics, Lasers, and Applications; 8785D7 (2013) https://doi.org/10.1117/12.2025878
Event: 8th Ibero American Optics Meeting/11th Latin American Meeting on Optics, Lasers, and Applications, 2013, Porto, Portugal

Abstract

A number of optical techniques have been reported in the scientific literature as accomplishable methodologies to diagnose diseases in biological tissue, for instance, diffuse reflectance spectroscopy (DRS) and optical polarization imaging (OPI). The skin is the largest organ in the body and consists of three primary layers, namely, the epidermis (the outermost layer exposed to the world), the dermis, and the hypodermis. The epidermis changes from to site to site, mainly because of difference in hydration. A lower water content increase light scattering and reduce the penetration depth of radiation. In this work, two hairless mice have been selected to evaluate their skin features by using DRS and OPI. Four areas of the specimen body were chosen to realize the comparison: back, abdomen, tail, and head. From DRS, it was possible to distinguish the skin nature because of different blood irrigation at dermis. In the other hand, OPI shows pseudo-depolarizing regions in the measured Mueller images related to a spatially varying propagation of the scattered light. This provides information about the cell size in the irradiated skin.

Citation Download Citation

A. Mora-Núñez, Y. Castillejos, G. García-Torales, and G. Martínez-Ponce "Diffuse reflectance spectroscopy and optical polarization imaging of in-vivo biological tissue", Proc. SPIE 8785, 8th Iberoamerican Optics Meeting and 11th Latin American Meeting on Optics, Lasers, and Applications, 8785D7 (18 November 2013); https://doi.org/10.1117/12.2025878

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KEYWORDS

Polarization

Tissue optics

Skin

Diffuse reflectance spectroscopy

Spectroscopy

Tissues

Polarimetry

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