High-resolution deep-tissue optical imaging using anti-Stokes phosphors

A. P. Popov; A. V. Karmenyan; A. V. Bykov; E. V. Khaydukov; A. V. Nechaev; O. A. Bibikova; V. Ya. Panchenko; V. A. Semchishen; V. N. Seminogov; A. S. Akhmanov; V. I. Sokolov; M. T. Kinnunen; V. V. Tuchin; A. V. Zvyagin

doi:10.1117/12.2032805

18 June 2013 High-resolution deep-tissue optical imaging using anti-Stokes phosphors

A. P. Popov, A. V. Karmenyan, A. V. Bykov, E. V. Khaydukov, A. V. Nechaev, O. A. Bibikova, V. Ya. Panchenko, V. A. Semchishen, V. N. Seminogov, A. S. Akhmanov, V. I. Sokolov, M. T. Kinnunen, V. V. Tuchin, A. V. Zvyagin

Author Affiliations +

Proceedings Volume 8801, Novel Biophotonic Techniques and Applications II; 88010C (2013) https://doi.org/10.1117/12.2032805
Event: European Conferences on Biomedical Optics, 2013, Munich, Germany

Abstract

We report on the high-resolution deep-tissue imaging using novel water-dispersible upconversion nanoparticles (UCNPs) β-NaYF₄:Yb^3+:Tm³⁺. Luminescence from the UCNP embedded into tissue-mimicking phantoms at the depth of 4 mm epi-illuminated with 975-nm laser radiation was detected. Fiber-optic detection shows 2-times better resolution compared with that obtained using CCD-based imaging modality. The conversion efficiency of upconversion particles and their cytotoxicity to HeLa cells were also investigated and reported.

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A. P. Popov, A. V. Karmenyan, A. V. Bykov, E. V. Khaydukov, A. V. Nechaev, O. A. Bibikova, V. Ya. Panchenko, V. A. Semchishen, V. N. Seminogov, A. S. Akhmanov, V. I. Sokolov, M. T. Kinnunen, V. V. Tuchin, and A. V. Zvyagin "High-resolution deep-tissue optical imaging using anti-Stokes phosphors", Proc. SPIE 8801, Novel Biophotonic Techniques and Applications II, 88010C (18 June 2013); https://doi.org/10.1117/12.2032805

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