Prognostic prospective of laser induced fluorescence as an objective tool to evaluate collagen deposition in thermal wounds: an ex vivo study

Vijendra Prabhu; Anusha Acharya; Satish Bola Sadashiva Rao; Pramod Kumar; Anurag Sharan; Krishna Kishore Mahato

doi:10.1117/12.2039416

4 March 2014 Prognostic prospective of laser induced fluorescence as an objective tool to evaluate collagen deposition in thermal wounds: an ex vivo study

Vijendra Prabhu, Anusha Acharya, Satish Bola Sadashiva Rao, Pramod Kumar, Anurag Sharan, Krishna Kishore Mahato

Author Affiliations +

Proceedings Volume 8926, Photonic Therapeutics and Diagnostics X; 892608 (2014) https://doi.org/10.1117/12.2039416
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

We aimed to investigate the predicative potential of laser induced fluorescence (LIF) in assessing collagen synthesis during wound healing progression following thermal injury. Granulation tissues of thermal wounds before grafting were collected and LIF measurements were carried out by exciting them with 325 nm to measure collagen. Biochemical estimation of the hydroxyproline was also carried out. Spectroscopic assessment & biochemical estimation of the wound granulation tissues displayed varied collagen levels. It can be concluded that LIF has the potential to be used as an objective tool to qualitatively determine the collagen levels in wound granulation tissues.

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Vijendra Prabhu, Anusha Acharya, Satish Bola Sadashiva Rao, Pramod Kumar, Anurag Sharan, and Krishna Kishore Mahato "Prognostic prospective of laser induced fluorescence as an objective tool to evaluate collagen deposition in thermal wounds: an ex vivo study", Proc. SPIE 8926, Photonic Therapeutics and Diagnostics X, 892608 (4 March 2014); https://doi.org/10.1117/12.2039416

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