Effect of LLLT on the level of ATP and ROS from organ of corti cells

ChungKu Rhee M.D.; So-Young Chang; Jin-Chul Ahn; Myung-Whan Suh; Jae Yun Jung

doi:10.1117/12.2041438

4 March 2014 Effect of LLLT on the level of ATP and ROS from organ of corti cells

ChungKu Rhee M.D., So-Young Chang, Jin-Chul Ahn, Myung-Whan Suh, Jae Yun Jung

Proceedings Volume 8926, Photonic Therapeutics and Diagnostics X; 89261Y (2014) https://doi.org/10.1117/12.2041438
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

It is well established that ototoxic antibiotics and acoustic trauma can damage cochlear hair cells and cause hearing loss. Previous studies using transcanal LLLT (Low level laser therapy) showed that LLLT can promote recovery of hearing thresholds and cochlear hair cells. However, its mechanism has not been studied. Aim: The aim of this study is to investigate the mechanism of hearing recovery from gentamicin induced ototoxic hearing loss by LLLT. Methods: HEI- OC1 (House ear institute organ of Corti) cells were cultured for 18 hours and ototoxicity was induced by gentamicin (GM) treatment to the cells. Cultured cells were divided into 6 groups, No treatment control, LLLT only, GM 6.6 mM and GM 13.1 mM, GM 6.6 mM+LLLT and GM 13.1 mM+LLLT cells. LD laser 808 nm, 15 mW, was irradiated to the cultured cells for 15 min, at 4 hours after GM treatment to the cells. ATP was assayed using the ATP assay Kit. ROS was measured using confocal microscope after application of H2DCFDA dye. Results: ATP was decreased in GM 13.1 mM cells and increased in LLLT only cells and GM 13.1 mM+LLLT cells compared to control and 13.1 mM cells. ROS was increased in GM 6.6 mM and GM 13.1 mM cells, and decreased in GM 6.6 mM+LLLT and GM 13.1 mM+LLLT cells compared to GM 6.6 and 13.1 mM cells immediately after laser irradiation. Conclusion: This study demonstrated that LLLT on GM treated HEI-OC1 cells increased ATP and decreased ROS that may contribute to the recovery of hearing.

Citation Download Citation

ChungKu Rhee M.D., So-Young Chang, Jin-Chul Ahn, Myung-Whan Suh, and Jae Yun Jung "Effect of LLLT on the level of ATP and ROS from organ of corti cells", Proc. SPIE 8926, Photonic Therapeutics and Diagnostics X, 89261Y (4 March 2014); https://doi.org/10.1117/12.2041438

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