Electrochemical impedance spectroscopy based-on interferon-gamma detection

Guan-Wei Li; Yi-Ching Kuo; Pei-I Tsai; Chih-Kung Lee

doi:10.1117/12.2039188

4 March 2014 Electrochemical impedance spectroscopy based-on interferon-gamma detection

Guan-Wei Li, Yi-Ching Kuo, Pei-I Tsai, Chih-Kung Lee

Proceedings Volume 8936, Design and Quality for Biomedical Technologies VII; 893606 (2014) https://doi.org/10.1117/12.2039188
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Tuberculosis (TB) is an ancient disease constituted a long-term menace to public health. According to World Health Organization (WHO), mycobacterium tuberculosis (MTB) infected nearly a third of people of the world. There is about one new TB occurrence every second. Interferon-gamma (IFN-γ) is associated with susceptibility to TB, and interferongamma release assays (IGRA) is considered to be the best alternative of tuberculin skin test (TST) for diagnosis of latent tuberculosis infection (LTBI). Although significant progress has been made with regard to the design of enzyme immunoassays for IFN-γ, adopting this assay is still labor-intensive and time-consuming. To alleviate these drawbacks, we used IFN-γ antibody to facilitate the detection of IFN-γ. An experimental verification on the performance of IGRA was done in this research. We developed two biosensor configurations, both of which possess high sensitivity, specificity, and rapid IFN-γ diagnoses. The first is the electrochemical method. The second is a circular polarization interferometry configuration, which incorporates two light beams with p-polarization and s-polarization states individually along a common path, a four photo-detector quadrature configuration to arrive at a phase modulated ellipsometer. With these two methods, interaction between IFN-γ antibody and IFN-γ were explored and presented in detail.

Citation Download Citation

Guan-Wei Li, Yi-Ching Kuo, Pei-I Tsai, and Chih-Kung Lee "Electrochemical impedance spectroscopy based-on interferon-gamma detection", Proc. SPIE 8936, Design and Quality for Biomedical Technologies VII, 893606 (4 March 2014); https://doi.org/10.1117/12.2039188

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available