Label-free biochemical characterization of bovine sperm cells using Raman microscopy

A. C. De Luca; S. Managò; M. A. Ferrara; L. Sirleto; R. Puglisi; D. Balduzzi; A. Galli; I. Rendina; P. Ferraro; G. Coppola

doi:10.1117/12.2039085

20 February 2014 Label-free biochemical characterization of bovine sperm cells using Raman microscopy

A. C. De Luca, S. Managò, M. A. Ferrara, L. Sirleto, R. Puglisi, D. Balduzzi, A. Galli, I. Rendina, P. Ferraro, G. Coppola

Author Affiliations +

Proceedings Volume 8938, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XIV; 893819 (2014) https://doi.org/10.1117/12.2039085
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

The current study relates to a Raman spectroscopy-based method for addressing the problem of sex assessment in mammals. A direct method for sex predetermination in animals is based on the X- and Y-bearing sperm cells sorting before insemination. Our Raman spectroscope allows distinguishing and characterizing the difference between X- and Y-bearing sperm cells by detecting and analyzing their Raman spectra in a non-invasive and non-destructive way.

Citation Download Citation

A. C. De Luca, S. Managò, M. A. Ferrara, L. Sirleto, R. Puglisi, D. Balduzzi, A. Galli, I. Rendina, P. Ferraro, and G. Coppola "Label-free biochemical characterization of bovine sperm cells using Raman microscopy", Proc. SPIE 8938, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XIV, 893819 (20 February 2014); https://doi.org/10.1117/12.2039085

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