Photothermal bleaching in time-lapse photoacoustic microscopy

Liang Gao; Lidai Wang; Chiye Li; Alejandro Garcia-Uribe; Lihong V. Wang

doi:10.1117/12.2038564

3 March 2014 Photothermal bleaching in time-lapse photoacoustic microscopy

Liang Gao, Lidai Wang, Chiye Li, Alejandro Garcia-Uribe, Lihong V. Wang

Proceedings Volume 8943, Photons Plus Ultrasound: Imaging and Sensing 2014; 89433G (2014) https://doi.org/10.1117/12.2038564
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

We studied the phenomenon of photothermal bleaching — a gradual reduction of contrast agent particles during repeated scans in photoacoustic microscopy. The dependence of the photothermal bleaching rate on the excitation pulse energy was determined while the laser focal diameter was held constant. Our results showed that, the dependence of the photothermal bleaching rate on the excitation pulse energy differed before and after the absorbers were raised to their melting point by the deposited laser energy. Based on this finding, we suggested an optimal excitation pulse energy, which balances the photothermal bleaching rate and signal amplitude, for time-lapse imaging applications.

Citation Download Citation

Liang Gao, Lidai Wang, Chiye Li, Alejandro Garcia-Uribe, and Lihong V. Wang "Photothermal bleaching in time-lapse photoacoustic microscopy", Proc. SPIE 8943, Photons Plus Ultrasound: Imaging and Sensing 2014, 89433G (3 March 2014); https://doi.org/10.1117/12.2038564

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