Development of tyrosinase-based reporter genes for preclinical photoacoustic imaging of mesenchymal stem cells

Julia Märk; Karen Ruschke; Hakan Dortay; Isabelle Schreiber; Andrea Sass; Taimoor Qazi; Matthias Pumberger; Jan Laufer

doi:10.1117/12.2039260

3 March 2014 Development of tyrosinase-based reporter genes for preclinical photoacoustic imaging of mesenchymal stem cells

Julia Märk, Karen Ruschke, Hakan Dortay, Isabelle Schreiber, Andrea Sass, Taimoor Qazi, Matthias Pumberger, Jan Laufer

Author Affiliations +

Proceedings Volume 8943, Photons Plus Ultrasound: Imaging and Sensing 2014; 89433Z (2014) https://doi.org/10.1117/12.2039260
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

The capability to image stem cells in vivo in small animal models over extended periods of time is important to furthering our understanding of the processes involved in tissue regeneration. Photoacoustic imaging is suited to this application as it can provide high resolution (tens of microns) absorption-based images of superficial tissues (cm depths). However, stem cells are rare, highly migratory, and can divide into more specialised cells. Genetic labelling strategies are therefore advantageous for their visualisation. In this study, methods for the transfection and viral transduction of mesenchymal stem cells with reporter genes for the co-expression of tyrosinase and a fluorescent protein (mCherry). Initial photoacoustic imaging experiments of tyrosinase expressing cells in small animal models of tissue regeneration were also conducted. Lentiviral transduction methods were shown to result in stable expression of tyrosinase and mCherry in mesenchymal stem cells. The results suggest that photoacoustic imaging using reporter genes is suitable for the study of stem cell driven tissue regeneration in small animals.

Citation Download Citation

Julia Märk, Karen Ruschke, Hakan Dortay, Isabelle Schreiber, Andrea Sass, Taimoor Qazi, Matthias Pumberger, and Jan Laufer "Development of tyrosinase-based reporter genes for preclinical photoacoustic imaging of mesenchymal stem cells", Proc. SPIE 8943, Photons Plus Ultrasound: Imaging and Sensing 2014, 89433Z (3 March 2014); https://doi.org/10.1117/12.2039260

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available