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The papers included in this volume were part of the technical conference cited on the cover and title page. Papers were selected and subject to review by the editors and conference program committee. Some conference presentations may not be available for publication. The papers published in these proceedings reflect the work and thoughts of the authors and are published herein as submitted. The publisher is not responsible for the validity of the information or for any outcomes resulting from reliance thereon. Please use the following format to cite material from this book: Author(s), “Title of Paper,” in Multiphoton Microscopy in the Biomedical Sciences XIV, edited by Ammasi Periasamy, Peter T. C. So, Karsten König, Proceedings of SPIE Vol. 8948 (SPIE, Bellingham, WA, 2014) Article CID Number. ISSN: 1605-7422 ISBN: 9780819498618 Published by SPIE P.O. Box 10, Bellingham, Washington 98227-0010 USA Telephone +1 360 676 3290 (Pacific Time) · Fax +1 360 647 1445 Copyright © 2014, Society of Photo-Optical Instrumentation Engineers. Copying of material in this book for internal or personal use, or for the internal or personal use of specific clients, beyond the fair use provisions granted by the U.S. Copyright Law is authorized by SPIE subject to payment of copying fees. The Transactional Reporting Service base fee for this volume is $18.00 per article (or portion thereof), which should be paid directly to the Copyright Clearance Center (CCC), 222 Rosewood Drive, Danvers, MA 01923. Payment may also be made electronically through CCC Online at copyright.com. Other copying for republication, resale, advertising or promotion, or any form of systematic or multiple reproduction of any material in this book is prohibited except with permission in writing from the publisher. The CCC fee code is 1605-7422/14/$18.00. Printed in the United States of America. Publication of record for individual papers is online in the SPIE Digital Library.
The CID Number appears on each page of the manuscript. The complete citation is used on the first page, and an abbreviated version on subsequent pages. Numbers in the index correspond to the last two digits of the six-digit CID Number. Conference CommitteeSymposium Chairs
Program Track Chairs
Conference Chairs
Conference Program Committee
Session Chairs
IntroductionMultiphoton microscopy has been established as the 3D imaging method of choice for studying biomedical specimens from single cells to whole animals with sub-micron resolution. Two decades have passed since the realization of two-photon laser scanning microscopy. The ever-expanding scope of applications and the continuing instrumental innovations require a forum where new ideas can be exchanged and presented. Our conference at the SPIE Photonics West BiOS 2014 meeting continues to address this need. This is the 14th year of this conference and we start our conference with three keynote lectures from leaders in the field: Drs. Martin Booth from University of Oxford (United Kingdom), 8948-1: Adaptive optics from microscopy to nanoscopy, Chris Xu, Cornell University (United States), 8948-2: In vivo deep tissue multiphoton imaging, and Karsten König, Saarland University (Germany), 8958-3: Quantitative multiphoton imaging. For the third year in a row, the conference is extremely pleased to have the JenLab Young Investigator Award, in addition to our regular poster awards. This award is donated by Dr. Karsten König, President and Founder of JenLab GmbH (Germany). The award selection committee includes Drs. Arnd Krueger (NewPort-Spectra Physics), Conor Evans (Harvard University), Paul Campagnola, University of Wisconsin-Madison, and the three conference chairs. The selection process includes the abstract, manuscript and poster presentation. Two finalists are selected for oral presentation after their poster presentation. The two finalists are (1) Dr. Gitanjal Deka, National Yang Ming University (Taiwan), Multiphoton microscopy for skin wound healing study in terms of cellular metabolism and collagen regeneration [8948-71], (2) Ms. Adi Schejter, Nairouz Farah, Shy Shoham, Technion-Israel Institute of Technology (Israel), Two-photon in vivo imaging of retinal microstructures [8948- 75]. Dr. Deka from National Yang Ming University (Taiwan) was selected as the winner of the JenLab Young Investigator Award 2014. For 14 years in a row, the conference organized poster awards for the students and postdoctoral fellows. The poster award was donated by all the conference sponsors including Becker & Hickl, Boston Electronics, Chroma Technology, Coherent, ISS, Newport-Spectra Physics, Princeton Instruments, Semrock, and Carl Zeiss. The three poster award winners are
Some of the most valuable contributions in this volume are articles written by highly experienced practitioners of multi-photon microscopy. They have enumerated the most important considerations in designing multi-photon microscopes and imaging experiments. Further, updates on the state-of-the-art commercial multi-photon microscope systems are presented. This volume also includes articles describing some recent advances in major multi-photon microscope components and applications including laser light sources, ultrafast optics, filters, FRET, FLIM, FCS, Raman, CARS, SRS and CRS microscopy and spectroscopy, single molecule, super-resolution imaging, endoscopy and various scientific and clinical applications. On a personal note, the Conference Chairs are grateful for the participation of all authors, session chairs and acknowledge the innovation-driven manufacturers (Becker & Hickl GmbH, Boston Electronics, Chroma Technology, Coherent, ISS, JenLab GmbH, Newport-Spectra Physics, Princeton Instruments, Semrock, and Carl Zeiss) for their enthusiastic support in organizing this conference successfully for the last 14 years. We look forward to other exciting conferences in the second decade and welcome your continued participation and support. Ammasi Periasamy Peter T. C. So Karsten König |
