Adaptive optics two photon microscopy with direct wavefront sensing using autofluorescent guide-stars

Xiaodong Tao; Andrew Norton; Matthew Kissel; Oscar Azucena; Joel Kubby

doi:10.1117/12.2044090

7 March 2014 Adaptive optics two photon microscopy with direct wavefront sensing using autofluorescent guide-stars

Xiaodong Tao, Andrew Norton, Matthew Kissel, Oscar Azucena, Joel Kubby

Proceedings Volume 8978, MEMS Adaptive Optics VIII; 89780D (2014) https://doi.org/10.1117/12.2044090
Event: SPIE MOEMS-MEMS, 2014, San Francisco, California, United States

Abstract

A fast direct wavefront sensing method for dynamic in-vivo adaptive optical two photon microscopy has demonstrated. By using the direct wavefront sensing and open loop control, the system provides high-speed wavefront measurement and correction. To measure the wavefront in the middle of a Drosophila embryo at early stages, autofluorescence from endogenous fluorophores in the yolk were used as reference guide-stars. This method does not rely on fluorescently labeled proteins as guide-stars, which can simplify the sample preparation for wavefront measurement. The method was tested through live imaging of a Drosophila embryo. The aberration in the middle of the embryo was measured directly for the first time. After correction, both contrast and signal intensity of the structure in the middle of the embryo was improved.

Citation Download Citation

Xiaodong Tao, Andrew Norton, Matthew Kissel, Oscar Azucena, and Joel Kubby "Adaptive optics two photon microscopy with direct wavefront sensing using autofluorescent guide-stars ", Proc. SPIE 8978, MEMS Adaptive Optics VIII, 89780D (7 March 2014); https://doi.org/10.1117/12.2044090

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