Assessment of neuroglial relationships under photodynamic treatment using fluorescent visualization of giant axons in crayfish ventral nerve cord

Eugeny Duz; Mikhail S. Kolosov

doi:10.1117/12.2049306

30 January 2014 Assessment of neuroglial relationships under photodynamic treatment using fluorescent visualization of giant axons in crayfish ventral nerve cord

Eugeny Duz, Mikhail S. Kolosov

Author Affiliations +

Proceedings Volume 9031, Saratov Fall Meeting 2013: Optical Technologies in Biophysics and Medicine XV; and Laser Physics and Photonics XV; 90310F (2014) https://doi.org/10.1117/12.2049306
Event: Saratov Fall Meeting 2013, 2013, Saratov, Russian Federation

Abstract

There are lots of publications last time about important role of neuroglial interactions in normal physiology of nervous system. Some of them infer that neuroglial interactions may support survival of neuronal and glial cells. Study the mechanisms of neuroglial interactions needs convenient models which are simple nervous system. They could be easily isolated and studied while retain natural neuroglial relations. The purpose of present work was to estimate glia protective properties of giant axons in simple model – crayfish ventral nerve cord, upon photooxidative stress. To better visualize the axons shape and reveal the periaxonal glial cells, we were filling of the axons with fluorescent label by means of specially devised microinjector. It enabled us to reveal the glial cells which are in close proximity to the axons. Such glial cells was found to have increased survival in compare to more distant ones from the axons under oxidative stress. The obtained data indicate that the crayfish giant axons have glia protective properties, which molecular mechanisms have to be studied.

Citation Download Citation

Eugeny Duz and Mikhail S. Kolosov "Assessment of neuroglial relationships under photodynamic treatment using fluorescent visualization of giant axons in crayfish ventral nerve cord", Proc. SPIE 9031, Saratov Fall Meeting 2013: Optical Technologies in Biophysics and Medicine XV; and Laser Physics and Photonics XV, 90310F (30 January 2014); https://doi.org/10.1117/12.2049306

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Axons

Glia

Nerve

Visualization

Microscopy

Fluorescent markers

Luminescence

Show All Keywords

Keywords/Phrases

Search In:

Publication Years