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A new technique for the visualization of small molecular components in living cells is presented. It is based on the exploitation, by means of video techniques of the slight diffraction power of small metal particles (20-40 nm) in the light microscope. We will show that colloidal gold probes, coupled to selective antibodies or charge modulating molecules allow to follow and quantify dynamic processes in endocytosis, Saltatory Motion and membrane mobility.
Rony Nuydens,Hugo Geerts,Marc De Brabander, andMarc Moeremans
"Dynamics Of Cell Components Investigated With Nanovid Microscopy", Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); https://doi.org/10.1117/12.945394
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Rony Nuydens, Hugo Geerts, Marc De Brabander, Marc Moeremans, "Dynamics Of Cell Components Investigated With Nanovid Microscopy," Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); https://doi.org/10.1117/12.945394