Multivariate optical element platform for compressed detection of fluorescence markers

Ryan J. Priore; Joseph A. Swanstrom

doi:10.1117/12.2053570

21 May 2014 Multivariate optical element platform for compressed detection of fluorescence markers

Ryan J. Priore, Joseph A. Swanstrom

Proceedings Volume 9101, Next-Generation Spectroscopic Technologies VII; 91010E (2014) https://doi.org/10.1117/12.2053570
Event: SPIE Sensing Technology + Applications, 2014, Baltimore, MD, United States

Abstract

The success of a commercial fluorescent diagnostic assay is dependent on the selection of a fluorescent biomarker; due to the broad nature of fluorescence biomarker emission profiles, only a small number of fluorescence biomarkers may be discriminated from each other as a function of excitation source. Multivariate Optical Elements (MOEs) are thin-film devices that encode a broad band, spectroscopic pattern allowing a simple broadband detector to generate a highly sensitive and specific detection for a target analyte. MOEs have historically been matched 1:1 to a discrete analyte or class prediction; however, MOE filter sets are capable of sensing projections of the original sparse spectroscopic space enabling a small set of MOEs to discriminate a multitude of target analytes. This optical regression can offer real-time measurements with relatively high signal-to-noise ratios that realize the advantages of multiplexed detection and pattern recognition in a simple optical instrument. The specificity advantage of MOE-based sensors allows fluorescent biomarkers that were once incapable of discrimination from one another via optical band pass filters to be employed in a common assay panel. A simplified MOE-based sensor may ultimately reduce the requirement for highly trained operators as well as move certain life science applications like disease prognostication from the laboratory to the point of care. This presentation will summarize the design and fabrication of compressed detection MOE filter sets for detecting multiple fluorescent biomarkers simultaneously with strong spectroscopic interference as well as comparing the detection performance of the MOE sensor with traditional optical band pass filter methodologies.

Citation Download Citation

Ryan J. Priore and Joseph A. Swanstrom "Multivariate optical element platform for compressed detection of fluorescence markers", Proc. SPIE 9101, Next-Generation Spectroscopic Technologies VII, 91010E (21 May 2014); https://doi.org/10.1117/12.2053570

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