Force dependence of phagosome trafficking in retinal pigment epithelial cells

Rebekah Daniel; Andrew T. Koll; David Altman

doi:10.1117/12.2062055

16 September 2014 Force dependence of phagosome trafficking in retinal pigment epithelial cells

Rebekah Daniel, Andrew T. Koll, David Altman

Proceedings Volume 9164, Optical Trapping and Optical Micromanipulation XI; 916435 (2014) https://doi.org/10.1117/12.2062055
Event: SPIE NanoScience + Engineering, 2014, San Diego, California, United States

Abstract

Retinal pigment epithelial (RPE) cells play an integral role in the renewal of photoreceptor disk membranes. As rod and cone cells shed their outer segments, they are phagocytosed and degraded by the RPE, and a failure in this process can result in retinal degeneration. We have studied the role of myosin VI in nonspecific phagocytosis in a human RPE primary cell line (ARPE-19), testing the hypothesis that this motor generates the forces required to traffic phagosomes in these cells. Experiments were conducted in the presence of forces through the use of in vivo optical trapping. Our results support a role for myosin VI in phagosome trafficking and demonstrate that applied forces modulate rates of phagosome trafficking.

Citation Download Citation

Rebekah Daniel, Andrew T. Koll, and David Altman "Force dependence of phagosome trafficking in retinal pigment epithelial cells", Proc. SPIE 9164, Optical Trapping and Optical Micromanipulation XI, 916435 (16 September 2014); https://doi.org/10.1117/12.2062055

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