Drug/protein interactions studied by time-resolved fluorescence spectroscopy

Thomas Gustavsson; Dimitra Markovitsi; Ignacio Vayá; Paula Bonancía; M. Consuelo Jiménez; Miguel A. Miranda

doi:10.1117/12.2063917

9 September 2014 Drug/protein interactions studied by time-resolved fluorescence spectroscopy

Thomas Gustavsson, Dimitra Markovitsi, Ignacio Vayá, Paula Bonancía, M. Consuelo Jiménez, Miguel A. Miranda

Proceedings Volume 9165, Physical Chemistry of Interfaces and Nanomaterials XIII; 91651E (2014) https://doi.org/10.1117/12.2063917
Event: SPIE NanoScience + Engineering, 2014, San Diego, California, United States

Abstract

We report here on a recent time-resolved fluorescence study [1] of the interaction between flurbiprofen (FBP), a chiral non-steroidal anti-inflammatory drug, and human serum albumin (HSA), the main transport protein in the human body. We compare the results obtained for the drug-protein complex with those of various covalently linked flurbiprofentryptophan dyads having well-defined geometries. In all cases stereoselective dynamic fluorescence quenching is observed, varying greatly from one system to another. In addition, the fluorescence anisotropy decays also display a clear stereoselectivity. For the drug-protein complexes, this can be interpreted in terms of the protein microenvironment playing a significant role in the conformational relaxation of FBP, which is more restricted in the case of the (R)- enantiomer.

Citation Download Citation

Thomas Gustavsson, Dimitra Markovitsi, Ignacio Vayá, Paula Bonancía, M. Consuelo Jiménez, and Miguel A. Miranda "Drug/protein interactions studied by time-resolved fluorescence spectroscopy", Proc. SPIE 9165, Physical Chemistry of Interfaces and Nanomaterials XIII, 91651E (9 September 2014); https://doi.org/10.1117/12.2063917

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