Label-free imaging of cellular malformation using high resolution photoacoustic microscopy

Zhongjiang Chen; Bingbing Li; Sihua Yang

doi:10.1117/12.2069106

17 September 2014 Label-free imaging of cellular malformation using high resolution photoacoustic microscopy

Zhongjiang Chen, Bingbing Li, Sihua Yang

Proceedings Volume 9230, Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014); 92300W (2014) https://doi.org/10.1117/12.2069106
Event: Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014), 2014, Wuhan, China

Abstract

A label-free high resolution photoacoustic microscopy (PAM) system for imaging cellular malformation is presented. The carbon fibers were used to testify the lateral resolution of the PAM. Currently, the lateral resolution is better than 2.7 μm. The human normal red blood cells (RBCs) were used to prove the imaging capability of the system, and a single red blood cell was mapped with high contrast. Moreover, the iron deficiency anemia RBCs were clearly distinguished from the cell morphology by using the PAM. The experimental results demonstrate that the photoacoustic microscopy system can accomplish label-free photoacoustic imaging and that it has clinical potential for use in the detection of erythrocytes and blood vessels malformation.

Citation Download Citation

Zhongjiang Chen, Bingbing Li, and Sihua Yang "Label-free imaging of cellular malformation using high resolution photoacoustic microscopy", Proc. SPIE 9230, Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014), 92300W (17 September 2014); https://doi.org/10.1117/12.2069106

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