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Photodynamic therapy (PDT) is a clinically approved treatment that was applied to oncology , dermatology, and ophthalmology. Reactive oxygen species (ROS) play a important role in the efficacy of PDT. Online monitoring of reactive oxygen species is the key to understand effect of PDT treatment. We used Fluorescence probes DPBF and luminescent probe luminal to measure the ROS in cells. And we revaluate the relationship between the amount of light and cell survival. There is strongly correlated between the amount of light and cell kill.
Weidong Gao,Yuanshu Zhou, andYueqing Gu
"Comparison of two strategies for detection of reactive oxygen species", Proc. SPIE 9230, Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014), 92301R (17 September 2014); https://doi.org/10.1117/12.2068769
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Weidong Gao, Yuanshu Zhou, Yueqing Gu, "Comparison of two strategies for detection of reactive oxygen species," Proc. SPIE 9230, Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014), 92301R (17 September 2014); https://doi.org/10.1117/12.2068769