Laser cross-linking protein captures for living cells on a biochip

Chih-Lang Lin; Ming-Jeng Pan; Hai-Wen Chen; Che-Kuan Lin; Chuen-Fu Lin; Patrice L. Baldeck

doi:10.1117/12.2079717

2 March 2015 Laser cross-linking protein captures for living cells on a biochip

Chih-Lang Lin, Ming-Jeng Pan, Hai-Wen Chen, Che-Kuan Lin, Chuen-Fu Lin, Patrice L. Baldeck

Proceedings Volume 9310, Frontiers in Biological Detection: From Nanosensors to Systems VII; 93100D (2015) https://doi.org/10.1117/12.2079717
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

In this study, bio-sensing pads are proposed to capture living cells, which are fabricated on cover glasses by cross-linking proteins/antibodies using laser induced photochemistry. The biological functions of the cross-linked protein/antibody were verified by capturing Staphylococcus aureus (S. aureus), Leptospira, and red blood cells (RBCs), separately, with associated protein/antibody sensing pads. The experimental results show that S. aureus were bound on GFP-AcmA’ pad after minutes of incubation and phosphate buffered saline (PBS) rinsing. No binding was observed with reference pad made of neutral bovine serum albumin (BSA). Second, A-type RBCs were chosen as the model cell to demonstrate the blood typing feasibility of the anti-A pad in microchannel. The A-type RBCs were captured only by the anti-A pad, but not the reference pad made of BSA. The same experimental model was carried out on the Leptospira, which stuck on the blood serum pad after PBS rinsing, but not BSA pad. This study provides a potential platform for simple and direct detection of living full cells without culture that could be used in point-of-care settings.

Citation Download Citation

Chih-Lang Lin, Ming-Jeng Pan, Hai-Wen Chen, Che-Kuan Lin, Chuen-Fu Lin, and Patrice L. Baldeck "Laser cross-linking protein captures for living cells on a biochip", Proc. SPIE 9310, Frontiers in Biological Detection: From Nanosensors to Systems VII, 93100D (2 March 2015); https://doi.org/10.1117/12.2079717

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available