One-micron resolution optical coherence tomography (OCT) in vivo for cellular level imaging

Dongyao Cui; Xinyu Liu; Jing Zhang; Xiaojun Yu; Ding Sun; Yuemei Luo; Jun Gu; Ping Shum; Linbo Liu

doi:10.1117/12.2076438

2 March 2015 One-micron resolution optical coherence tomography (OCT) in vivo for cellular level imaging

Dongyao Cui, Xinyu Liu, Jing Zhang, Xiaojun Yu, Ding Sun, Yuemei Luo, Jun Gu, Ping Shum, Linbo Liu

Proceedings Volume 9312, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIX; 93123R (2015) https://doi.org/10.1117/12.2076438
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

We developed a spectral domain OCT system combining two NIR, CW light sources of different spectral range. Its resolving power is validated by visualizing the cellular structures of zebra fish larvae in vivo. An NIR extended illumination from 755-1100 nm is achieved. The axial resolution is 1.27 μm in air, corresponding to 0.93μm in tissue (n=1.36), which is the highest axial resolution using NIR, CW laser sources up to date to the best of our knowledge. In vivo imaging is conducted to demonstrate the resolving power of proposed one-micron resolution OCT system. The top and bottom surfaces of individual disk-like red blood cell is reliably visualized, as well as flat, spindle shaped endothelial cells lining along the luminal surface of the blood vessel wall. This study provides a viable solution for cellular and subcellular level OCT imaging system which is also very competitive in cost.

Citation Download Citation

Dongyao Cui, Xinyu Liu, Jing Zhang, Xiaojun Yu, Ding Sun, Yuemei Luo, Jun Gu, Ping Shum, and Linbo Liu "One-micron resolution optical coherence tomography (OCT) in vivo for cellular level imaging", Proc. SPIE 9312, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIX, 93123R (2 March 2015); https://doi.org/10.1117/12.2076438

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KEYWORDS

Cameras

Optical coherence tomography

In vivo imaging

Signal to noise ratio

Image resolution

Imaging systems

Mirrors

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