Photo-imprint super-resolution photoacoustic microscopy

Junjie Yao; Lidai Wang; Chiye Li; Chi Zhang; Lihong V. Wang

doi:10.1117/12.2077350

11 March 2015 Photo-imprint super-resolution photoacoustic microscopy

Junjie Yao, Lidai Wang, Chiye Li, Chi Zhang, Lihong V. Wang

Proceedings Volume 9323, Photons Plus Ultrasound: Imaging and Sensing 2015; 932327 (2015) https://doi.org/10.1117/12.2077350
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Combining the absorption-based photoacoustic effect and intensity-dependent photobleaching effect, we demonstrate a simple method for super-resolution photoacoustic imaging of both fluorescent and non-fluorescent samples. Our method is based on a double-excitation process, where the first excitation pulse partially and inhomogeneously bleaches the molecules in the diffraction-limited excitation volume, thus biasing the signal contributions from a second excitation pulse striking the same region. By scanning the excitation beam, we performed three-dimensional sub-diffraction imaging of varied fluorescent and non-fluorescent species. A lateral resolution of 80 nm and an axial resolution of 370 nm have been demonstrated. This technique has the potential to enable label-free super-resolution imaging, and can be transferred to other optical imaging modalities or combined with other super-resolution methods.

Citation Download Citation

Junjie Yao, Lidai Wang, Chiye Li, Chi Zhang, and Lihong V. Wang "Photo-imprint super-resolution photoacoustic microscopy", Proc. SPIE 9323, Photons Plus Ultrasound: Imaging and Sensing 2015, 932327 (11 March 2015); https://doi.org/10.1117/12.2077350

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