A folic acid labelled carbon quantum dot-protoporphryin IX conjugate for use in folate receptor targeted two-photon excited photodynamic therapy

Dean Nicholas; Colin Fowley; Anthony P. McHale; Sukanta Kamila; Jason Sheng; Jordan Atchison; John F. Callan

doi:10.1117/12.2084821

12 March 2015 A folic acid labelled carbon quantum dot-protoporphryin IX conjugate for use in folate receptor targeted two-photon excited photodynamic therapy

Dean Nicholas, Colin Fowley, Anthony P. McHale, Sukanta Kamila, Jason Sheng, Jordan Atchison, John F. Callan

Author Affiliations +

Proceedings Volume 9338, Colloidal Nanoparticles for Biomedical Applications X; 933813 (2015) https://doi.org/10.1117/12.2084821
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Folic acid (FA) has been used as a molecular targeting strategy to improve the specificity of a CQD-protoporphyrin IX (CQD-PPIX) conjugate to folate receptor positive (FR+) HeLa cells for use in two-photon excited Photodynamic Therapy (TPE-PDT). FA was covalently attached to the CQD-PPIX conjugate to form a FA-CQD-PPIX conjugate. The uptake of the FA-CQD-PPIX conjugate in FR+ HeLa cells was shown to be 7 times greater than the CQD-PPIX conjugate, while both conjugates showed a similar uptake in FR negative (FR-) HT-47 cells. TPE-PDT experiments, using HeLa cells as a target, revealed a 30% improved cytotoxicity for cells treated with the FA-CQD-PPIX conjugate and TPE compared to controls treated with the CQD-PPIX conjugate and TPE. Collectively, these results suggest the presence of FA can facilitate targeting of CQD-sensitiser conjugates to FR+ cells resulting in an improved PDT effect.

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Dean Nicholas, Colin Fowley, Anthony P. McHale, Sukanta Kamila, Jason Sheng, Jordan Atchison, and John F. Callan "A folic acid labelled carbon quantum dot-protoporphryin IX conjugate for use in folate receptor targeted two-photon excited photodynamic therapy", Proc. SPIE 9338, Colloidal Nanoparticles for Biomedical Applications X, 933813 (12 March 2015); https://doi.org/10.1117/12.2084821

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