Microfabrication of three-dimensional filters for liposome extrusion

Tommaso Baldacchini; Vicente Nuñez; Christopher N. LaFratta; Joseph S. Grech; Valentine I. Vullev; Ruben Zadoyan

doi:10.1117/12.2083903

16 March 2015 Microfabrication of three-dimensional filters for liposome extrusion

Tommaso Baldacchini, Vicente Nuñez, Christopher N. LaFratta, Joseph S. Grech, Valentine I. Vullev, Ruben Zadoyan

Proceedings Volume 9353, Laser 3D Manufacturing II; 93530W (2015) https://doi.org/10.1117/12.2083903
Event: SPIE LASE, 2015, San Francisco, California, United States

Abstract

Liposomes play a relevant role in the biomedical field of drug delivery. The ability of these lipid vesicles to encapsulate and transport a variety of bioactive molecules has fostered their use in several therapeutic applications, from cancer treatments to the administration of drugs with antiviral activities. Size and uniformity are key parameters to take into consideration when preparing liposomes; these factors greatly influence their effectiveness in both in vitro and in vivo experiments. A popular technique employed to achieve the optimal liposome dimension (around 100 nm in diameter) and uniform size distribution is repetitive extrusion through a polycarbonate filter. We investigated two femtosecond laser direct writing techniques for the fabrication of three-dimensional filters within a microfluidics chip for liposomes extrusion. The miniaturization of the extrusion process in a microfluidic system is the first step toward a complete solution for lab-on-a-chip preparation of liposomes from vesicles self-assembly to optical characterization.

Citation Download Citation

Tommaso Baldacchini, Vicente Nuñez, Christopher N. LaFratta, Joseph S. Grech, Valentine I. Vullev, and Ruben Zadoyan "Microfabrication of three-dimensional filters for liposome extrusion", Proc. SPIE 9353, Laser 3D Manufacturing II, 93530W (16 March 2015); https://doi.org/10.1117/12.2083903

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