Determination of type and concentration of DNA nitrogenous bases by Raman spectroscopy

Kirill A. Laptinskiy; Sergey Burikov; Tatiana Dolenko

doi:10.1117/12.2180055

19 March 2015 Determination of type and concentration of DNA nitrogenous bases by Raman spectroscopy

Kirill A. Laptinskiy, Sergey Burikov, Tatiana Dolenko

Proceedings Volume 9448, Saratov Fall Meeting 2014: Optical Technologies in Biophysics and Medicine XVI; Laser Physics and Photonics XVI; and Computational Biophysics; 94480J (2015) https://doi.org/10.1117/12.2180055
Event: Saratov Fall Meeting 2014, 2014, Saratov, Russian Federation

Abstract

In this paper results of application of laser Raman spectroscopy for increasing reliability of molecular DNA computations are presented. It is shown that elaborated method provides the accuracy of determination of concentration of individual nitrogenous bases 0.03 g/l and the accuracy of determination of total DNA concentration in the solutions 0.04 g/l by Raman spectra.

Citation Download Citation

Kirill A. Laptinskiy, Sergey Burikov, and Tatiana Dolenko "Determination of type and concentration of DNA nitrogenous bases by Raman spectroscopy", Proc. SPIE 9448, Saratov Fall Meeting 2014: Optical Technologies in Biophysics and Medicine XVI; Laser Physics and Photonics XVI; and Computational Biophysics, 94480J (19 March 2015); https://doi.org/10.1117/12.2180055

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