Photodynamic injury of isolated crayfish neuron and surrounding glial cells: the role of p53

S. A. Sharifulina; A. B. Uzdensky

doi:10.1117/12.2179569

19 March 2015 Photodynamic injury of isolated crayfish neuron and surrounding glial cells: the role of p53

S. A. Sharifulina, A. B. Uzdensky

Proceedings Volume 9448, Saratov Fall Meeting 2014: Optical Technologies in Biophysics and Medicine XVI; Laser Physics and Photonics XVI; and Computational Biophysics; 94480L (2015) https://doi.org/10.1117/12.2179569
Event: Saratov Fall Meeting 2014, 2014, Saratov, Russian Federation

Abstract

The pro-apoptotic transcription factor p53 is involved in cell responses to injurious impacts. Using its inhibitor pifithrin- α and activators tenovin-1, RITA and WR-1065, we studied its potential participation in inactivation and death of isolated crayfish mechanoreceptor neuron and satellite glial cells induced by photodynamic treatment, a strong inducer of oxidative stress. In dark, p53 activation by tenovin-1 or WR-1065 shortened activity of isolated neurons. Tenovin-1 and WR-1065 induced apoptosis of glial cells, whereas pifithrin-α was anti-apoptotic. Therefore, p53 mediated glial apoptosis and suppression of neuronal activity after axotomy. Tenovin-1 but not other p53 modulators induced necrosis of axotomized neurons and surrounding glia, possibly, through p53-independent pathway. Under photodynamic treatment, p53 activators tenovin-1 and RITA enhanced glial apoptosis indicating the pro-apoptotic activity of p53. Photoinduced necrosis of neurons and glia was suppressed by tenovin-1 and, paradoxically, by pifithrin-α. Modulation of photoinduced changes in the neuronal activity and necrosis of neurons and glia was possibly p53-independent. The different effects of p53 modulators on neuronal and glial responses to axotomy and photodynamic impact were apparently associated with different signaling pathways in neurons and glial cells.

Citation Download Citation

S. A. Sharifulina and A. B. Uzdensky "Photodynamic injury of isolated crayfish neuron and surrounding glial cells: the role of p53", Proc. SPIE 9448, Saratov Fall Meeting 2014: Optical Technologies in Biophysics and Medicine XVI; Laser Physics and Photonics XVI; and Computational Biophysics, 94480L (19 March 2015); https://doi.org/10.1117/12.2179569

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