High-contrast imaging of mycobacterium tuberculosis using third-harmonic generation microscopy

Bo Ram Kim; Eungjang Lee; Seung-Han Park

doi:10.1117/12.2187632

8 July 2015 High-contrast imaging of mycobacterium tuberculosis using third-harmonic generation microscopy

Bo Ram Kim, Eungjang Lee, Seung-Han Park

Proceedings Volume 9523, International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015; 95230G (2015) https://doi.org/10.1117/12.2187632
Event: International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015, 2015, Jeju, Korea, Republic of

Abstract

Nonlinear optical microcopy has become an important tool in investigating biomaterials due to its various advantages such as label-free imaging capabilities. In particular, it has been shown that third-harmonic generation (THG) signals can be produced at interfaces between an aqueous medium (e.g. cytoplasm, interstitial fluid) and a mineralized lipidic surface. In this work, we have demonstrated that label-free high-contrast THG images of the mycobacterium tuberculosis can be obtained using THG microscopy.

Citation Download Citation

Bo Ram Kim, Eungjang Lee, and Seung-Han Park "High-contrast imaging of mycobacterium tuberculosis using third-harmonic generation microscopy", Proc. SPIE 9523, International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015, 95230G (8 July 2015); https://doi.org/10.1117/12.2187632

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