PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
We report multiplexed spectral imaging of plasmon resonance shifts in a simple setup, capable of resolving single protein binding events with a high signal to noise ratio of up to 10 for single Fibronectin (450 kDa) proteins. We directly record 2-dimensional, spectrally dispersed images of multiple gold nanorods. The spectra are corrected via an overlapped particle image that correlates arbitrary particle positions to the reference slit position, allowing us to use the full 2D space of the sensor. Together with the simple experimental implementation, our approach paves the way towards routine and efficient label-free detection down to the single molecule level.
Aleksandar Sebesta,Alexandar Weigel, andPhilipp Kukura
"Multiplexed label-free detection of single proteins", Proc. SPIE 9554, Nanoimaging and Nanospectroscopy III, 95541D (26 August 2015); https://doi.org/10.1117/12.2188277
ACCESS THE FULL ARTICLE
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
The alert did not successfully save. Please try again later.
Aleksandar Sebesta, Alexandar Weigel, Philipp Kukura, "Multiplexed label-free detection of single proteins," Proc. SPIE 9554, Nanoimaging and Nanospectroscopy III, 95541D (26 August 2015); https://doi.org/10.1117/12.2188277