Monitoring longitudinal changes in irradiated head and neck cancer xenografts using diffuse reflectance spectroscopy

Karthik Vishwanath; Shudong Jiang; Jason R. Gunn; Kayla Marra; Jacqueline Marie Andreozzi; Brian W. Pogue

doi:10.1117/12.2213192

29 February 2016 Monitoring longitudinal changes in irradiated head and neck cancer xenografts using diffuse reflectance spectroscopy

Karthik Vishwanath, Shudong Jiang, Jason R. Gunn, Kayla Marra, Jacqueline Marie Andreozzi, Brian W. Pogue

Author Affiliations +

Proceedings Volume 9689, Photonic Therapeutics and Diagnostics XII; 96891W (2016) https://doi.org/10.1117/12.2213192
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Radiation therapy is often used as the preferred clinical treatment for control of localized head and neck cancer. However, during the course of treatment (6-8 weeks), feedback about functional and/or physiological changes within impacted tissue are not obtained, given the onerous financial and/or logistical burdens of scheduling MRI, PET or CT scans. Diffuse optical sensing is well suited to address this problem since the instrumentation can be made low-cost and portable while still being able to non-invasively provide information about vascular oxygenation in vivo. Here we report results from studies that employed an optical fiber-based portable diffuse reflectance spectroscopy (DRS) system to longitudinally monitor changes in tumor vasculature within two head and neck cancer cell lines (SCC-15 and FaDu) xenografted in the flanks of nude mice, in two separate experiments. Once the tumor volumes were 100mm³, 67% of animals received localized (electron beam) radiation therapy in five fractions (8Gy/day, for 5 days) while 33% of the animals served as controls. DRS measurements were obtained from each animal on each day of treatment and then for two weeks post-treatment. Reflectance spectra were parametrized to extract total hemoglobin concentration and blood oxygen-saturation and the resulting time-trends of optical parameters appear to be dissimilar for the two cell-lines. These findings are also compared to previous animal experiments (using the FaDu line) that were irradiated using a photon beam radiotherapy protocol. These results and implications for the use of fiber-based DRS measurements made at local (irradiated) tumor site as a basis for identifying early radiotherapy-response are presented and discussed.

Citation Download Citation

Karthik Vishwanath, Shudong Jiang, Jason R. Gunn, Kayla Marra, Jacqueline Marie Andreozzi, and Brian W. Pogue "Monitoring longitudinal changes in irradiated head and neck cancer xenografts using diffuse reflectance spectroscopy", Proc. SPIE 9689, Photonic Therapeutics and Diagnostics XII, 96891W (29 February 2016); https://doi.org/10.1117/12.2213192

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