Assessing the effect of a high-fat diet on rodents' adipose tissue using Brillouin and Raman spectroscopy

Maria Troyanova-Wood; Cassidy Gobbell; Zhaokai Meng; Vladislav V. Yakovlev

doi:10.1117/12.2213525

7 March 2016 Assessing the effect of a high-fat diet on rodents' adipose tissue using Brillouin and Raman spectroscopy

Maria Troyanova-Wood, Cassidy Gobbell, Zhaokai Meng, Vladislav V. Yakovlev

Proceedings Volume 9703, Optical Biopsy XIV: Toward Real-Time Spectroscopic Imaging and Diagnosis; 970310 (2016) https://doi.org/10.1117/12.2213525
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

The purpose of this study is to evaluate the effect of a high-lipid diet on elasticity of adipose tissue. We employed dual Raman/Brillouin microspectroscopy to analyze brown and white adipose tissues obtained from adult rats. The rats were divided into two groups, one of which received a high-fat feed, while the other served as a control. We hypothesized that the changes in the elasticity of adipose tissues between the two groups can be successfully assessed using Brillouin spectroscopy. We found that the brown adipose tissue possessed a lesser Brillouin shift than the white adipose within each group and that the elastic modulus of both adipose tissues increases in the high-fat diet group. The Raman spectra provided supplementary chemical information and indicated an increase in the lipid-to-protein ratio in the brown adipose, but not in the white adipose.

Conference Presentation

Citation Download Citation

Maria Troyanova-Wood, Cassidy Gobbell, Zhaokai Meng, and Vladislav V. Yakovlev "Assessing the effect of a high-fat diet on rodents' adipose tissue using Brillouin and Raman spectroscopy", Proc. SPIE 9703, Optical Biopsy XIV: Toward Real-Time Spectroscopic Imaging and Diagnosis, 970310 (7 March 2016); https://doi.org/10.1117/12.2213525

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available