7 March 2022Monitoring the delivery of lipid nanoparticle encapsulated self-amplifying mRNA vaccine using coherent Raman microscopy and multiphoton imaging
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Self-amplifying mRNA (SAM), a synthetic RNA vaccine which self-replicates upon delivery into the cytoplasm encapsulated with lipid nanoparticles (LNPs), leads to a strong and sustained immune response. In this study, we investigated SAM-LNP uptake and subsequent SAM release and distribution in baby hamster kidney (BHK-21) cells using coherent anti-Stokes Raman scattering (CARS) and multiphoton imaging techniques. This work demonstrates the significance of multimodal imaging techniques to capture the successful delivery of SAM and the subsequent production of proteins within cells. Our study can be further extended to label-free detection techniques to investigate targeted drug-delivery.
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Kajari Bera, Prabuddha Mukherjee, Edita Aksamitiene, Aneesh Alex, Jindou Shi, Darold R. Spillman Jr., Marina Marjanovic, Ahmed Shabana, Russell Johnson, Steve R. Hood, Stephen A. Boppart, "Monitoring the delivery of lipid nanoparticle encapsulated self-amplifying mRNA vaccine using coherent Raman microscopy and multiphoton imaging," Proc. SPIE PC11938, Visualizing and Quantifying Drug Distribution in Tissue VI, PC119380A (7 March 2022); https://doi.org/10.1117/12.2609087