Flexible miniature 3-photon micro-endoscope using a functionnalized hollow core fiber

Dylan Septier; Gaelle Brevalle; Géraud Bouwmans; Rémi Habert; Damien Labat; Andy Cassez; Karen Baudelle; Vasyl Mytskaniuk; Hervé Rigneault; Alexandre Kudlinski

doi:10.1117/12.2614426

9 March 2022 Flexible miniature 3-photon micro-endoscope using a functionnalized hollow core fiber

Dylan Septier, Gaelle Brevalle, Géraud Bouwmans, Rémi Habert, Damien Labat, Andy Cassez, Karen Baudelle, Vasyl Mytskaniuk, Hervé Rigneault, Alexandre Kudlinski

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Proceedings Volume PC11949, Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XX; PC119490L (2022) https://doi.org/10.1117/12.2614426
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

We demonstrate 3-photon fluorescence micro-endoscopy using a negative curvature hollow core fiber and a 2.2 mm miniature scanning head. The fiber design allows distortion-less, delivery of <100 fs pulses without dispersion pre-compensation requirements. The fiber also features a double cladding allowing the back-collection of nonlinear signals through the same fiber. Sub-micron spatial resolution together with large field of view is made possible by the combination of a miniature distal objective lens with a functionnalization of the fiber output with a GRIN fiber spliced to the output facet. 3-photon fluoresence imaging is demonstrated on various biological samples.

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Dylan Septier, Gaelle Brevalle, Géraud Bouwmans, Rémi Habert, Damien Labat, Andy Cassez, Karen Baudelle, Vasyl Mytskaniuk, Hervé Rigneault, and Alexandre Kudlinski "Flexible miniature 3-photon micro-endoscope using a functionnalized hollow core fiber", Proc. SPIE PC11949, Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XX, PC119490L (9 March 2022); https://doi.org/10.1117/12.2614426

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KEYWORDS

Imaging systems

Brain

Neuroimaging

Image resolution

In vivo imaging

Luminescence

Multiphoton fluorescence microscopy

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