Nanodiamond based phantom for assessing multimodal fluorescence and multiharmonic imaging systems

Mantas Žurauskas; Aneesh Alex; Jaena Park; Steve Hood; Stephen A. Boppart

doi:10.1117/12.2608258

7 March 2022 Nanodiamond based phantom for assessing multimodal fluorescence and multiharmonic imaging systems

Mantas Žurauskas, Aneesh Alex, Jaena Park, Steve Hood, Stephen A. Boppart

Proceedings Volume PC11951, Design and Quality for Biomedical Technologies XV; PC1195103 (2022) https://doi.org/10.1117/12.2608258
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Characterizing the performance of fluorescence microscopy and nonlinear imaging systems is an essential step required for imaging system optimization and quality control during longitudinal experiments. Emerging multimodal nonlinear imaging techniques require a new generation of microscopy calibration targets that are not susceptible to bleaching, and can provide a contrast across the multiple modalities. Here, we present a nanodiamond-based calibration target for microscopy, designed for facilitating reproducible measurements at the object plane. Since fluorescent nanodiamonds are not prone to bleaching shelf-stable sample can provide a rapid reference measurements for ensuring consistent performance of microscopy systems in microscopy laboratories and imaging facilities.

Conference Presentation

Citation Download Citation

Mantas Žurauskas, Aneesh Alex, Jaena Park, Steve Hood, and Stephen A. Boppart "Nanodiamond based phantom for assessing multimodal fluorescence and multiharmonic imaging systems", Proc. SPIE PC11951, Design and Quality for Biomedical Technologies XV, PC1195103 (7 March 2022); https://doi.org/10.1117/12.2608258

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