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7 March 2022 Virtual hematoxylin and eosin histopathology with ultraviolet photoacoustic remote sensing and synthetic aperture optical coherence microscopy
Matthew T. Martell, Nathaniel J. M. Haven, Brendon S. Restall, Sveta Silverman, Lashan Peiris, Jean Deschenes, Roger J. Zemp
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Proceedings Volume PC11960, Photons Plus Ultrasound: Imaging and Sensing 2022; PC1196006 (2022) https://doi.org/10.1117/12.2610470
Event: SPIE BiOS, 2022, San Francisco, California, United States
Abstract
A combined synthetic aperture optical coherence microscopy and ultraviolet photoacoustic remote sensing system is presented, capable of fast scanning of tissues for tumor margin inspection. It provides a fast 3D OCT mode for imaging tissues to depths of ~1mm, and a superficial virtual histology mode provided by absorption contrast UV-PARS for virtual hematoxylin contrast and coherence-gated scattering microscopy for virtual eosin contrast. Breast lumpectomy specimens are scanned in each mode to evaluate the extent of features in depth and generate en-face images with histological detail and realism, providing results accurately interpretable by pathologists.
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Matthew T. Martell, Nathaniel J. M. Haven, Brendon S. Restall, Sveta Silverman, Lashan Peiris, Jean Deschenes, and Roger J. Zemp "Virtual hematoxylin and eosin histopathology with ultraviolet photoacoustic remote sensing and synthetic aperture optical coherence microscopy", Proc. SPIE PC11960, Photons Plus Ultrasound: Imaging and Sensing 2022, PC1196006 (7 March 2022); https://doi.org/10.1117/12.2610470
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KEYWORDS
Optical coherence tomography
Photoacoustic spectroscopy
Remote sensing
Ultraviolet radiation
Image resolution
Microscopy
Optical coherence microscopy
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