Large field of view functional photoacoustic remote sensing microscopy using a stabilized temperature-regulated stimulated Raman scattering light source

Lyazzat Mukhangaliyeva; Zohreh Hosseinaee; Benjamin Ecclestone; Nicholas Pellegrino; Marian Boktor; Layla Khalili; Paul Fieguth; Parsin Haji Reza

doi:10.1117/12.2609910

7 March 2022 Large field of view functional photoacoustic remote sensing microscopy using a stabilized temperature-regulated stimulated Raman scattering light source

Lyazzat Mukhangaliyeva, Zohreh Hosseinaee, Benjamin Ecclestone, Nicholas Pellegrino, Marian Boktor, Layla Khalili, Paul Fieguth, Parsin Haji Reza

Author Affiliations +

Proceedings Volume PC11960, Photons Plus Ultrasound: Imaging and Sensing 2022; PC119601G (2022) https://doi.org/10.1117/12.2609910
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

We present a new technique for improving the temporal stability of the Stimulated Raman Scattering (SRS)-based multispectral pulsed source by decreasing the temperature of the SRS medium. This technique reduces temporal fluctuations of the output SRS peaks, generates stable multiwavelength light, improves repeatability and accuracy of functional measurements. This stabilized temperature-regulated SRS-based source is combined with the wide field of view photoacoustic remote sensing microscope utilizing a telecentric scan lens as an imaging objective. In-vivo functional imaging experiments of the chorioallantois membrane of a chicken embryo (CAM) are performed for validation purposes.

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Lyazzat Mukhangaliyeva, Zohreh Hosseinaee, Benjamin Ecclestone, Nicholas Pellegrino, Marian Boktor, Layla Khalili, Paul Fieguth, and Parsin Haji Reza "Large field of view functional photoacoustic remote sensing microscopy using a stabilized temperature-regulated stimulated Raman scattering light source", Proc. SPIE PC11960, Photons Plus Ultrasound: Imaging and Sensing 2022, PC119601G (7 March 2022); https://doi.org/10.1117/12.2609910

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