OCT-guided contour scanning for dynamically-focused photoacoustic remote sensing microscopy

Matthew T. Martell; Nathaniel J. M. Haven; Sveta Silverman; Lashan Peiris; Jean Deschenes; Roger J. Zemp

doi:10.1117/12.2610457

7 March 2022 OCT-guided contour scanning for dynamically-focused photoacoustic remote sensing microscopy

Matthew T. Martell, Nathaniel J. M. Haven, Sveta Silverman, Lashan Peiris, Jean Deschenes, Roger J. Zemp

Proceedings Volume PC11960, Photons Plus Ultrasound: Imaging and Sensing 2022; PC119601K (2022) https://doi.org/10.1117/12.2610457
Event: SPIE BiOS, 2022, San Francisco, California, United States

Conference Poster

Abstract

Ultraviolet photoacoustic remote sensing (UV-PARS) microscopy is a non-contact imaging modality capable of producing label-free absorption contrast images of cell nuclei. This virtual hematoxylin-like imaging combined with virtual eosin-like data from 1310 nm scattering microscopy can provide complete virtual H&E histologically in unstained tissues. Here, we develop contour scanning for applying UV-PARS and scattering-based virtual histology in fresh and formalin-fixed thick tissues. Our spectral-domain OCT-guided approach initially scans specimens in 3D, and a custom algorithm extracts the surface contour. A high-resolution UV-PARS scan is then performed using a z-axis stage for dynamic focusing to compensate for sample surface irregularities.

Conference Presentation

Citation Download Citation

Matthew T. Martell, Nathaniel J. M. Haven, Sveta Silverman, Lashan Peiris, Jean Deschenes, and Roger J. Zemp "OCT-guided contour scanning for dynamically-focused photoacoustic remote sensing microscopy", Proc. SPIE PC11960, Photons Plus Ultrasound: Imaging and Sensing 2022, PC119601K (7 March 2022); https://doi.org/10.1117/12.2610457

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