Non-destructive label-free multispectral fluorescence lifetime imaging microscopy for live imaging of tumor spheroids

Jeongmoo Han; Joonha Park; Soonyong Kwon; Jaesang Kim; Ungyo Kang; Jessie Sungyun Jeon; Bomi Gweon; Hongki Yoo

doi:10.1117/12.2610340

7 March 2022 Non-destructive label-free multispectral fluorescence lifetime imaging microscopy for live imaging of tumor spheroids

Jeongmoo Han, Joonha Park, Soonyong Kwon, Jaesang Kim, Ungyo Kang, Jessie Sungyun Jeon, Bomi Gweon, Hongki Yoo

Author Affiliations +

Proceedings Volume PC11964, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XX; PC1196402 (2022) https://doi.org/10.1117/12.2610340
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

We developed a label-free fluorescence lifetime imaging microscopy (FLIM) to quantify the metabolic state of tumor spheroids. We obtained label-free 3D FLIM images from different focal plane of cancer (MCF7) spheroids for 4 different spectral bands which was segregated by the custom-made spectral resolving unit. To obtain optically sectioned images, we used an optical fiber instead of a spatial filter. Statistical analyses showed that the signals acquired by FLIM—fluorescence intensity and lifetime—reflected well the metabolic state of cancer cells constituting spheroids. These results demonstrated the potential for quantitative measurement of the cellular metabolism in a label-free, non-destructive manner.

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Jeongmoo Han, Joonha Park, Soonyong Kwon, Jaesang Kim, Ungyo Kang, Jessie Sungyun Jeon, Bomi Gweon, and Hongki Yoo "Non-destructive label-free multispectral fluorescence lifetime imaging microscopy for live imaging of tumor spheroids", Proc. SPIE PC11964, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XX, PC1196402 (7 March 2022); https://doi.org/10.1117/12.2610340

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KEYWORDS

Fluorescence lifetime imaging

Mode conditioning cables

3D image processing

Microscopy

Multispectral imaging

Stereoscopy

Cancer

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