Evaluating drug impact on human gut microbiome by SRS-FISH

Xiaowei Ge; Fátima C. Pereira; Haonan Lin; Michael Wagner; Ji-xin Cheng

doi:10.1117/12.2609993

2 March 2022 Evaluating drug impact on human gut microbiome by SRS-FISH

Xiaowei Ge, Fátima C. Pereira, Haonan Lin, Michael Wagner, Ji-xin Cheng

Proceedings Volume PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022; PC1197318 (2022) https://doi.org/10.1117/12.2609993
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

The gut microbiome has a considerable impact on human health. Furthermore, it is very sensitive to the in vivo environment changes. Entacapone (ENT) is one important human-targeted drug for the treatment of Parkinson’s disease shown to largely impact the growth of individual organisms in the gut microbiome. To resolve ENT incorporation and impact on gut microbiome activity in situ, we employed stimulated Raman scattering - fluorescence in situ hybridization (SRS-FISH) along with ultrafast delay line tuning. The hyperspectral chemical images acquired with this method could better preserve and separate the chemical information, thus enabled high-throughput identity-specific single cell metabolism analysis and mapping of drug incorporation by individual microbiome cells.

Conference Presentation

Citation Download Citation

Xiaowei Ge, Fátima C. Pereira, Haonan Lin, Michael Wagner, and Ji-xin Cheng "Evaluating drug impact on human gut microbiome by SRS-FISH", Proc. SPIE PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022, PC1197318 (2 March 2022); https://doi.org/10.1117/12.2609993

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