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17 March 2023 Longitudinal high-resolution 4D imaging of the mouse embryonic heart development with optical coherence tomography
Andre C. Faubert, Irina V. Larina, Shang Wang
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Proceedings Volume PC12355, Diagnostic and Therapeutic Applications of Light in Cardiology 2023; PC123550C (2023) https://doi.org/10.1117/12.2649559
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
Understanding mammalian cardiogenesis requires high-resolution imaging to assess changes in cardiac structure, morphology, dynamics, and function during embryonic development. Optical coherence tomography (OCT) shows promise for advanced studies due to superior imaging scale and speed for 4D imaging of the early mouse embryonic heart. Building on this, and enabled by prolonged embryo culture, efficient reconstruction, and multi-dimensional visualization strategy, we present the first longitudinal, 4D, dual-contrast OCT imaging of the beating mouse embryonic heart over development for up to ~12 hours, termed as 5D cardiodynamic and hemodynamic imaging. This paves the way to study early developmental dynamics of the heart.
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Andre C. Faubert, Irina V. Larina, and Shang Wang "Longitudinal high-resolution 4D imaging of the mouse embryonic heart development with optical coherence tomography", Proc. SPIE PC12355, Diagnostic and Therapeutic Applications of Light in Cardiology 2023, PC123550C (17 March 2023); https://doi.org/10.1117/12.2649559
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KEYWORDS
Heart
Optical coherence tomography
Confocal microscopy
Mouse models
Coherence (optics)
Hemodynamics
Light emitting diodes
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